Results: We expressed the PHD fusion protein in P. pastoris; furthermore, the PHD fused protein was purified to near homogeneity by DEAE Sepharose FF, Phenyl Sepharose HP and Blue Sepharose 6 FF. Our result showed that the increase of pentobarbital-induced hypnotic effect characterized by reducing sleep latency and prolonged sleep duration was observed for increasing concentrations of PHD fusion protein (P<0.05); moreover, different dose of PHD fusion protein could induce the mice to re-sleep in a dose-dependent manner, whereas higher doses of PHD fusion protein (1.0, 2.0 mg/kg) significantly increased the rate of sleep re-onset compared with the vehicle group of mice (P<0.05).

Conclusion: PHD fusion protein increased the hypnotic effects of pentobarbital by reducing sleep latency and prolonged sleep duration. The present study suggested PHD fusion protein could be a new drug candidate for insomnia.

Keywords: Delta sleep-inducing peptide; Pichia pastoris; fusion protein; human serum albumin; protein transduction domain.

Significance: Our study demonstrates that improvement of sleep quality by p-DSIP restores spatial memory by up regulating CREB phosphorylation during simulated high altitude hypoxia.

Source: Phosphorylated delta sleep inducing peptide restores spatial memory and p-CREB expression by improving sleep architecture at high altitude

Koustav Roy ¹, Garima Chauhan ¹, Punita Kumari ¹, Meetu Wadhwa ¹, Shahnawaz Alam ¹, Koushik Ray ¹, Usha Panjwani ¹, Krishna Kishore ²

Affiliations Expand

PMID: 30107169 https://pubmed.ncbi.nlm.nih.gov/30107169/